Surveillance and analysis of bacterial resistance in Huizhou from 2009 to 2010

OBJECTIVE To investigate the resistance of clinical isolates obtained from hospitals in Huizhou to commonly used antimicrobial agents.METHODS A total of 6 hospitals were involved in this program.Bacterial susceptibility testing was performed according to a unified protocol using Kirby-Bauer method.Results were analyzed according to CLSI 2009.The results were statistically analyzed with WHONET 5.4 software.RESULTS A total of 11105 pathogenic strains were clinically isolated in these hospitals from Jan.to Dec.2009.3044(27.41%) and 8061(72.59%) of which were gram-positive cocci and gram-negative bacilli respectively.The constitutional ratios of methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococcus were 35.8% and 88.1%,respectively.vancomycin and linezolid resistant gram-positive cocci were not found.The detection rates of extended spectrum beta lactamases in Escherichia coli and Klebsiella pneumonia isolates were 32.6% and 30.0%,respectively.Among non-fermentative gram-negative bacilli,the resistance rates of Pseudomonas aeruginosa to cefoperazone/sulbactam and piperacillin/tazuobatan were 7.5% and 9.2% respectively.Acinetobacter baumannii was susceptible to cefoperazone/sulbactam and Imipenem,with drug-resistance rate of 5.3% and 14.5% respectively.The resistance rate of Stenotrophomonas maltophilia to Cefoperazone/Sulbactam was 6.9%.CONCLUSION Regular surveillance of antimicrobial resistance in clinical isolates is important to understand the trend of antimicrobial resistance and will provide a theoretical basis for rational antimicrobial therapy.