Investigation on antimicrobial resistance of bacteria isolated from a hospital from 2004 to 2005

Objective To investigate the bacterial distribution and antimicrobial resistance of clinical isolates from a hospital between January 2004 and December 2005.Methods Bacterial were isolated and identified by routine method,antimicrobial susceptibility tests of clinical isolates were performed using Kirby-Bauer method.Results Of 2 220 clinical isolates,gram-negative and gram-positive bacteria accounted for 1 310(59.01%) and 910(40.99%)respectively.ESBLs-producing strains accounted for 39.03% and 38.33% in Escherichia coli and Klebsiella pneumoniae.Imipenem and meropenem were the most potent antimicrobial agents against strains of Enterobacteriaceae.In the nonfermentative gram-negative bacilli,resistant rate of Pseudomonas aeruginosa to imipenem and meropenem was 27.86% and 29.66% respectively.Stenotrophomonas maltophilia was sensitive to cefoperazone-sulbactam and SMZ-TMP,drug-resistant rate was 45.15% and 17.74% respectively.In Staphylococcus aureus and coagulase negative Staphylococcus,MRSA and MRCNS accounted for 60.48% and 44.21% respectively,but all were sensitive to vancomycin.No Enterococcus faecalis and Enterococcus faecium strains resistant to vancomycin has been found.Conclusion The resistance of clinical isolates from the hospital is serious,so it is necessary to choose antimicrobial agents in the treatment of infection caused by pathogenic bacteria.