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2006•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Gallic Acid in 21-component Hanshuishi Powder by HPLC

Lin Pengcheng

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Abstract

ObjectiveTo establish a quantitative method to determine the content of gallic acid in 21-component Hanshuishi Powder.MethodsThe content of gallic acid in 21-component Hanshuishi powder was determined by HPLC.The separation was performed on Kromasil C_(18) column with a mobile phase of methanol-water(5∶95) and detected at wavelength of 274 nm.ResultsThe linearity of the method was good at range of 0.020 6～0.247 2 μg and the average recovery of gallic acid was 99.1%,RSD was 1.3%.ConclusionThe method is convenient,accurate,and can be used for the determination of gallic acid in 21-component Hanshuishi Powder.

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What this paper is about

ObjectiveTo establish a quantitative method to determine the content of gallic acid in 21-component Hanshuishi Powder.MethodsThe content of gallic acid in 21-component Hanshuishi powder was determined by HPLC.The separation was performed on Kromasil C_(18) column with a mobile phase of methanol-water(5∶95) and detected at wavelength of 274 nm.ResultsThe linearity of the method was good at range of 0.020 6～0.247 2 μg and the average recovery of gallic acid was 99.1%,RSD was 1.3%.ConclusionThe method is convenient,accurate,and can be used for the determination of gallic acid in 21-component Hanshuishi Powder.

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Available abstract

ObjectiveTo establish a quantitative method to determine the content of gallic acid in 21-component Hanshuishi Powder.MethodsThe content of gallic acid in 21-component Hanshuishi powder was determined by HPLC.The separation was performed on Kromasil C_(18) column with a mobile phase of methanol-water(5∶95) and detected at wavelength of 274 nm.ResultsThe linearity of the method was good at range of 0.020 6～0.247 2 μg and the average recovery of gallic acid was 99.1%,RSD was 1.3%.ConclusionThe method is convenient,accurate,and can be used for the determination of gallic acid in 21-component Hanshuishi Powder.

Key concepts: Gallic acid, High-performance liquid chromatography, Chromatography, Chemistry, Content determination, Methanol, Organic chemistry, Antioxidant

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