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2006•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Gallic Acid in Baixuan Xiatare Capsule by HPLC

Xiangqun Jin

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Abstract

ObjectiveAn HPLC method is developed for the determination of gallic acid in Baixuan Xiatare capsule.MethodsThe separation was performed on Diamonsil-C_(18) column with a mobile phase of MeOH-0.1%H_3PO_4(7∶93) and detected at wavelength of 270 nm.ResultsThe linearity range was 0.012 5～0.062 5 μg(r=0.999 1,n=5) and the average recovery of gallic acid was 99.52%,RSD was 0.56%.ConclusionThe method is simple,sensitive and reproducible.It can be used in quality control of Baixuan Xiatare Capsule.

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What this paper is about

ObjectiveAn HPLC method is developed for the determination of gallic acid in Baixuan Xiatare capsule.MethodsThe separation was performed on Diamonsil-C_(18) column with a mobile phase of MeOH-0.1%H_3PO_4(7∶93) and detected at wavelength of 270 nm.ResultsThe linearity range was 0.012 5～0.062 5 μg(r=0.999 1,n=5) and the average recovery of gallic acid was 99.52%,RSD was 0.56%.ConclusionThe method is simple,sensitive and reproducible.It can be used in quality control of Baixuan Xiatare Capsule.

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Available abstract

ObjectiveAn HPLC method is developed for the determination of gallic acid in Baixuan Xiatare capsule.MethodsThe separation was performed on Diamonsil-C_(18) column with a mobile phase of MeOH-0.1%H_3PO_4(7∶93) and detected at wavelength of 270 nm.ResultsThe linearity range was 0.012 5～0.062 5 μg(r=0.999 1,n=5) and the average recovery of gallic acid was 99.52%,RSD was 0.56%.ConclusionThe method is simple,sensitive and reproducible.It can be used in quality control of Baixuan Xiatare Capsule.

Key concepts: Gallic acid, Capsule, High-performance liquid chromatography, Chromatography, Chemistry, Organic chemistry, Biology, Antioxidant

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