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2009•Anhui Medical and Pharmaceutical JournalRequires access

Determination of gallic acid in tankejing tablets by HPLC

Yan Liu

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Abstract

Aim To establish a method for determining gallic acid in tankejing tablets.Methods HPLC method was used.The samples were separated by Kromasil ODS column(250 mm×4.6 mm,5 μm).The mobile phase was water,methanol,phosphate(95∶5∶0.05).The detection wavelength was at 273 nm,with a flow rate at 1.0 ml·min-1.The column temperature was 35℃.Results The linear range of gallic acid was from 0.856 to 4.280 mg·L-1.The average recovery was 99.18%,and RSD was 1.38%.Conclusion The method is accurate,simple,specific,and can be used for quality control of gallic acid in Tankejing tablets.

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What this paper is about

Aim To establish a method for determining gallic acid in tankejing tablets.Methods HPLC method was used.The samples were separated by Kromasil ODS column(250 mm×4.6 mm,5 μm).The mobile phase was water,methanol,phosphate(95∶5∶0.05).The detection wavelength was at 273 nm,with a flow rate at 1.0 ml·min-1.The column temperature was 35℃.Results The linear range of gallic acid was from 0.856 to 4.280 mg·L-1.The average recovery was 99.18%,and RSD was 1.38%.Conclusion The method is accurate,simple,specific,and can be used for quality control of gallic acid in Tankejing tablets.

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Available abstract

Aim To establish a method for determining gallic acid in tankejing tablets.Methods HPLC method was used.The samples were separated by Kromasil ODS column(250 mm×4.6 mm,5 μm).The mobile phase was water,methanol,phosphate(95∶5∶0.05).The detection wavelength was at 273 nm,with a flow rate at 1.0 ml·min-1.The column temperature was 35℃.Results The linear range of gallic acid was from 0.856 to 4.280 mg·L-1.The average recovery was 99.18%,and RSD was 1.38%.Conclusion The method is accurate,simple,specific,and can be used for quality control of gallic acid in Tankejing tablets.

Key concepts: Gallic acid, Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Content determination, Methanol, Phosphate buffered saline

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