Determination of Gallic Acid in the Xiaoyankangjun Tablets by HPLC

OBJECTIVE To establish a HPLC method for determining the gallic acid content in the Xiaoyankangjun tablets.METHODS Aglient ZORBAXSB-C18 column(4.6 mm×250 mm,5 μm) was used,the mobile phase consisted of methanol-0.025 mol·L-1 phosphoric aqueous solution(10∶90);the flow rate was 0.6 mL·min-1 and column temperature was set at room temperature;the detecting wavelength was set at 215 nm. RESULTS The method was linear with the range of 1.168~23.36 μg·mL-1 for gallic acid(r=0.999 5);the average recovery was 99.44%;the RSD was 1.10%.CONCLUSION The method is simple,rapid and accurate.It can be used for quantitative determination of gallic acid in the Xiaoyankangjun tablets.