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Study on HPLC fingerprint of Rhizoma Belamcandae

Zhang Yuanyuan, Minjian Qin, Pan Shu, Li Lu

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Abstract

Objective:To study the HPLC fingerprints and establish a sensitive and specific method for the quality control of Rhizoma Belameandae from different origins.Methods:The samples were separated on an Agilent Zorbax SB-C_(18) column(4.6 mm×250 mm,5μm) with the mobile phase consisting of acetonitrile and 0.4%acetic acid water solution by gradient elution.The flow rate was 1 mL·min~(-1);wavelength was 269 nm.Results:There were 14 common peaks in the HPLC fingerprints of 15 samples.By comparision of the retention time of chemical standards,7 peaks were identified. Conclusions:Although the HPLC fingerprints of the Rhizoma Belamcandae from different origins were varied slightly,tire established HPLC fingerprints can be used for the quality control of Rhizoma Belamcandae.

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What this paper is about

Objective:To study the HPLC fingerprints and establish a sensitive and specific method for the quality control of Rhizoma Belameandae from different origins.Methods:The samples were separated on an Agilent Zorbax SB-C_(18) column(4.6 mm×250 mm,5μm) with the mobile phase consisting of acetonitrile and 0.4%acetic acid water solution by gradient elution.The flow rate was 1 mL·min~(-1);wavelength was 269 nm.Results:There were 14 common peaks in the HPLC fingerprints of 15 samples.By comparision of the retention time of chemical standards,7 peaks were identified. Conclusions:Although the HPLC fingerprints of the Rhizoma Belamcandae from different origins were varied slightly,tire established HPLC fingerprints can be used for the quality control of Rhizoma Belamcandae.

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Available abstract

Objective:To study the HPLC fingerprints and establish a sensitive and specific method for the quality control of Rhizoma Belameandae from different origins.Methods:The samples were separated on an Agilent Zorbax SB-C_(18) column(4.6 mm×250 mm,5μm) with the mobile phase consisting of acetonitrile and 0.4%acetic acid water solution by gradient elution.The flow rate was 1 mL·min~(-1);wavelength was 269 nm.Results:There were 14 common peaks in the HPLC fingerprints of 15 samples.By comparision of the retention time of chemical standards,7 peaks were identified. Conclusions:Although the HPLC fingerprints of the Rhizoma Belamcandae from different origins were varied slightly,tire established HPLC fingerprints can be used for the quality control of Rhizoma Belamcandae.

Key concepts: Chromatography, High-performance liquid chromatography, Gradient elution, Chemistry, Fingerprint (computing), Acetic acid, Acetonitrile, Biochemistry

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