HPLC-UV fingerprint of Cimicifugae Rhizoma from different regions

Objective To detect the HPLC-UV fingerprints of Cimicifugae Rhizoma. Methods The column of Waters XBridge® Shield RP18 (4.6 mm × 250 mm, 5 μm) and a mixture of 0.05% phosphoric acid water solution -acetonitrile as the mobile phase was at the flow rate of 1.0 ml/min, the column temperature was 30 ℃ and the ahsorhance was monitored at 320 nm, the injection volume was 20 μl. The fingerprints of Cimicifugae Rhizoma were analyzed, and the similarity analysis was used to evaluate the quality of Cimicifugae Rhizoma in different areas. Results To establish a method of HPLC-UV fingerprint of Cimicifugae Rhizoma, and eleven common peaks were identified of cimifugin, caffeic acid, isoferulic acid peaks. The similarity of the fingerprints of 12 batches of Cimicifugae Rhizoma in four producing areas was above 0.88. The precision, repeatability and stability of the method were good. Conclusions The method is simple, accurate and reproducible, which provides the basis for scientific evaluation of the quality control of Cimicifugae Rhizoma. Key words: Cimicifuga heracleifolia; Chromatography, high pressure liquid; Fingerprint; Quality control