Simultaneous determination of aloe-emodin,rhein,emodin,physcion,chrysophanol,baicalin,wogonin,berberine hydrochloride in Yiqing granules by HPLC

Objective To establish an HPLC method for simultaneous determination of aloe-emodin,rhein,emodin,physcion,chrysophanol,baicalin,wogonin,berberine hydrochloride in Yiqing granules(traditional chinese medicine). Methods Isolation of sample was achieved on a Kromasil C18 column(250 mm×4.6 mm,5 μm).A mixture of acetonitrile and 0.1% phosphoric acid(adjust pH=3.0 with triethylamine)with gradient elution was used as mobile phase.The analytes was detected at 254 nm,and the column temperature was maintained at 30 ℃. Results The linearity ranges of aloe-emodin,rhein,emodin,physcion,chrysophanol,baicalin,wogonin,berberine hydrochloride were 1.800-36.20 mg·L-1,14.25-285.1 mg·L-1,4.500-90.0 mg·L-1,1.780-35.60 mg·L-1,4.300-85.0 mg·L-1,48.20-963 mg·L-1,1.700-34.00 mg·L-1 and 28.60-572.5 mg·L-1,respectively(r≥0.999 1).The average recoveries(n=9)of them were 96.0%,98.0%,96.6%,97.0%,97.7%,97.0%,97.2% and 98.0%,respectively. Conclusions The method is fast,simple,sensitive and can be used for the quality control of Yiqing granules.