Simultaneous Determination of Notoginsenoside and Ginsenosides in Compound Naoluoxintong Extracts by High-performance Liquid Chromatography

Objective To establish a high-performance liquid chromatography(HPLC)method for simultaneous determination of notoginsenoside R1 and ginsenosides Rb1,Rg1,Rd,and Re in compound Naoluoxintong extracts and to provide a methodological basis for the quality control of compound Naoluoxintong.Methods HPLC was performed on an Welch Materials C8column(4.6mm ×250mm,5μm)with a mobile phase of acetonitrile-water for gradient elution at a flow rate of 1.2ml/min,a detection wavelength of 203 nm,and a column temperature of 20 ℃.Results The content of notoginsenoside R1 and ginsenosides Rb1,Rg1,Rd,and Re showed a good linear relationship with the peak area within 0.081 7-0.980 4μg(r=0.999 9),0.315 7-3.788 4μg(r=0.999 9),0.300 0-3.600 0μg(r=0.999 6),0.077 7-0.932 4μg(r=0.999 9),and 0.074 6-0.895 2μg(r=0.999 9);the average recovery rates were 99.72%,99.65%,101.23%,99.38%,and 100.62%(n=6).Conclusion The established HPLC method is simple,convenient,accurate,reliable,and repeatable.With this method,notoginsenoside R1 and ginsenosides Rb1,Rg1,Rd,and Re can be well separated,and this method can be used for the quality control of compound Naoluoxintong.