Determination of five saponins compounds in compound Xueshuantong capsule by HPLC

Objective To establish an HPLC method for determination of Notoginsenoside R1,Ginsenosides Rg1,Ginsenosides Re,Ginsenosides Rb1,Ginsenosides Rd in Compound Xueshuantong Capsule by HPLC. Methods The compounds were separated by gradient elution on YMC-Pack Pro C18column( 250 mm × 4. 6 mm,5 μm) as stationary phase; acetonitrile and water were adopted as gradient mixed mobile phase. The detection wavelength was set at 203 nm and the column temperature was 35℃,with the flow rate of 1. 0 m L·min- 1. Results Notoginsenoside R1,Ginsenosides Rg1,Ginsenosides Re,Ginsenosides Rb1,Ginsenosides Rd all had good linear relation( r = 0. 999 9) in the ranges of 50. 10 ~ 501. 00 ng,151. 02 ~ 1 510. 20 ng,32. 01 ~ 302. 10 ng,102. 23 ~ 1 022. 30 ng,16. 32 ~163. 20 ng,respectively. RSD of precision,stability and repeatability were lower than 3 %. The average recovery rates were in the range of 95% ~ 105%. Conclusions The method is simple,accurate,reproducible and suitable for the quality control of Compound Xueshuantong Capsule.