Determination of ginsenoside Rg_1 and notoginsenoside R_1 in Jiuweigantai capsule by UPLC

Objective To establish an UPLC method for the content determination of ginsenoside Rg1 and notoginsenoside R1 in Jiuweigantai capsule. Methods ACQUITY BEH C18 analytical column(2.1 mm×50 mm,1.7 μm) was used with the mobile phase consisting of acetonitrile-water in gradient elution at a flow rate of 0.4 ml/min.The detection wavelength was 203 nm,column temperature was 35℃,and injection volume was 1 μl. Results Ginsenoside Rg1and notoginsenoside R1 in Jiuweigantai capsule had good linearity within the range of 0.1-1.5 mg/ml and 0.04-0.6 mg/ml respectively,and their recoveries were 99.4%,101.8%,with RSD of 0.5%,1.3% respectively. Conclusion The method is convenient,quick,correct for determination of ginsenoside Rg1 and notoginsenoside R1 in Jiuweigantai capsule,and can be used for the quality control for Jiuweigantai capsule.