Study on in vitro Culture and Plant Regeneration of Milletia speciosa

Using seeds and stems with buds of wild Milletia speciosa as material,the effects of different hormone combination and explants on the in vitro culture were investigated.The result showed that the optimum treatment time of 1 g/L HgCl2 for seed and stem was 10 and 15 min respectively.The best hormone combination for callus and adventitious buds induction was 1.5 mg/L 6-BA+0.5 mg/L NAA+0.3 mg/L IBA,the highest callus induction rate of seeds and adventitious buds of stem with a bud was 95.8% and 45.8%,respectively.The best medium for differentiation of adventitious bud was 2.0 mg/L 6-BA +0.5 mg/L NAA;the differentiation rate was 58.3%.And the best rooting medium was 1/2MS+1.5 mg/L NAA+1.0 mg/L IBA;the rooting rate was 70.8%.The best matrix was mixture of equivalent volume of loess,peat and river sand,the survival rate of 90 days after transplantation reached 73.3%.