Screening conditions of adventitious bud induction and plantlet regeneration for Cunninghamia lanceolata.

【Objective】Conditions of the adventitious bud induction and plant regeneration for Cunninghamia lanceolata were screened and rapid propagation techniques for Chinese fir in Guangxi was explored to provide references for supply and tissue culture of Chinese fir seedlings. 【Method】Using base branches of superior Chinese fir species as testing materials, stem explant disinfection, adventitious bud induction and plant regeneration were the focal points in the current research. 【Result】 (1)The best disinfection method was treating stem explants with 75% ethanol for 30 s and 0.1% mercuric chloride for 6 min, which showed 30% contamination rate;(2)In the initial medium (1/2MS + NAA 0.2 mg/L + 6-BA 0.6 mg/L), the buds germinated on the 8 th day with the induction rate of 47%;(3)In the optimum subculture medium (1/2MS + IBA 0.3 mg/L + 6-BA 0.6 mg/L + white sugar 30 g/L), axillary buds were multiplied by 3.4 times on the 25 th day;(4)The induction effect was the best with the rooting rate of 52% in the rooting medium (1/4MS + IBA 0.15 mg/L + NAA 0.075 mg/L). 【Conclusion】It was beneficial to the adventitious bud induction for Chinese fir with the initial medium (1/2MS + NAA 0.2 mg/L + 6-BA 0.6 mg/L) and the optimum subculture medium (1/2MS + IBA 0.3 mg/L + 6-BA 0.6 mg/L + white sugar 30 g/L). NAA was conducive to rooting in vitro , and rooting induction effect was the best in the rooting medium of 1/4MS + IBA 0.15 mg/L + NAA 0.075 mg/L.