Study on Tissue Culture Techniques for Stem Segment of Millettia speciosa Champ.

[Objective] The study was to provide the basis for the scale production of Millettia speciosa Champ.and protect its wild resource.[Method] With tender stem segments of M.speciosa as explants,the techniques of its tissue culture and rapid propagation in vitro were studied.[Result] The explants were treated by 0.1% HgCl2 for 20 min and the asepsis material could be obtained.With MS+6-BA 1.0 mg/L+ IBA 0.1 mg/L as the initial medium,the induction rate could reach 100%.After inoculation for 20 d,the axillary buds begun to germinate and normally grow.With MS+6-BA 2.0 mg/L+ IBA 0.1 mg/L as the multiplication medium,after culture for 30 d,the multiplication coefficient reached 7.0,and there were a few callus emerged,but the growth and development of adventitious buds were normal.When the medium of 1/2 MS+ IBA 0.5 mg/L+ IAA 0.5 mg/L was used for rooting culture,the rooting effect was the best and the rooting rate reached 80%,and the growth of root was good.When the rooted small seeding were transplanted into the matrix of coconut bran and sand(3∶1),and the plastic film was covered on it for keeping moisture for 15 d,the survival rate reached more than 85%.[Conclusion] The optimum rooting medium for stem segments culture of M.speciosa was 1/2 MS+ IBA 0.5 mg/L+ IAA 0.5 mg/L.