Research on resveratrol's effect in suppressing growth and inducing apoptosis of C6 glioma cells

Objective To explore the contribution of resveratrol(Res)to the proliferation and apoptosis of brain glioma C6 cell line as well as fibroblast 3T3 cell line in vitro.Methods Methyl thiazolyl tetrazolium (MTT) assay was used to measure its effects on the proliferation of C6 and 3T3 cells cultured with different concentrations of Res for 24 h, 48 h, 72 h and 96 h. HE staining, scanning electron microscope(SEM), TUNEL fluorescence staining and the Annexin V assay by flow cytometer(FCM) were applied to detect the apoptosis induced by Res.Results Res obviously suppressed the proliferation( P 0.01) and induced the apoptosis of C6 rather than 3T3 in concentration and time dependent manner, meanwhile, as for C6, the rate of apoptosis was 29.7% , 14.6% after having been treated with 210 μmol/L and 120 μmol/L Res for 24 h, respectively. The rate of apoptosis in control was 2.1%. In contrast, the corresponding rate for 3T3 was 4.3%, 3.5% and 2.6%. Conclusion The results of this study confirm the ability of Res to suppress the proliferation of brain glioma C6 cells with a typical apoptotic feature in vitro. Therefore, Res might be considered as a possible treatment strategy for brain gliomas.