Experimental study on resveratrol suppressing the growth of SHG-44 glioma cells

Objective To explore the contribution of resveratrol (Res) to the proliferation and apoptosis of brain glioma SHG-44 cells in vitro.Methods Methyl thiazolyl tetrazolium(MTT)assay was used to measure its effects on the proliferation of SHG-44 cells cultured with different concentrations of Res for 6 h, 24 h, and 48 h. HE staining and Hoechst 33342 fluorescence staining were used to observe the cellular morphological changes. DNA ladder assay was used to detect the schizolysis of cellular DNA, the Annexin V-fluorescein isothiocyanate (FITC)and propidium iodide(PI) assay by flow cytometer(FCM) were applied to detect the apoptosis induced by Res. The changes of cell cycle were detected by FCM.Results Res obviously suppressed the proliferation of SHG-44 glioma cells (P0.01) in a concentration- and time-dependent manner. Res induced cellular apoptosis of the SHG-44 cells in a concentration-dependent manner, and mainly with an accumulation of G_ 1 and a reduction in the G_ 2 , S phase of the cell cycle.Conclusion Resveratrol significantly suppresses the growth of glioma cells, induces the apoptosis and changes the cell cycle of SHG-44 glioma cells. Therefore resveratrol may be considered as a possible treatment medicine for gliomas.