An empirical study of the inhibitory effect of resveratrol on the proliferation of MIAPaCa-2 cell in vitro

Objective:To explore the effect of resveratrol(Res) on the proliferation of pancreatic carcinoma MIAPaCa-2 cells in vitro and explore the rudimental mechanism. Methods: Methyl thiazolyl tetrazolium method (MTT) was used to detect the cell growth status of MIAPaCa-2 cells after treatment with Res. The morphologic changes of cells after treatment with Res were detected by inverted microscope and transmission electron microscope. The apoptosis rates were analyzed with flowcytometry(FCM) after the cells were treated with 25μmol·L-1, 50μmol·L-1, 100μmol·L-1 and 200μmol·L-1 Res for 48 h, respectively. Results: Res inhibited the proliferation of MIAPaCa-2 cells in a concentration-and time-dependent manner as measured by MTT method. After the cells were treated with Res, inverted microscope observed the reduction of the cell number and found significant morphologic changes, characterized by cell rounding and cell nuclear enrichment suggesting apoptosis. And the presence of typical morphological changes of apoptosis was confirmed with transmission electron microscope. The apoptosis rates at 25μmol·L-1, 50 μmol·L-1, 100μmol·L-1 and 200μmol·L-1 Res-treated groups after treatment for 48 h, measured by FCM, were significantly higher than those in control groups(P0.05). Conclusion: These findings suggest that the natural product Res may have a potent anti-proliferative effect on MIAPaCa-2 cells,the mechanisms of which may be related to the induction of apoptosis.