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2010•ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSISRequires access

A Comparative Study on Different Methods for Extraction of Gallus Genomic DNA

Ma XinHong, Kang JuanJuan, Kang Xiang-tao, Han Rui-li, Sun Guirong, Jun Pan, Huang HuAng, Kai Liu, Liheng Zhang

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Abstract

The optimized cooking extraction method was used in this study to extract gallus genomic DNA.OD260 /OD280 was up to 1.77±0.06 by the SmartspecTMplus test.The result showed that the quality of DNA extracted by the optimized cooking extraction method was very good,clear and bright fragments were detected through PCR amplification.Compared with the Phenol-chloroform extraction method,The optimized cooking extraction method is free from chloroform,phenol and other toxic reagents,and saves time and costs,hence a good method for extracting genomic DNA.

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What this paper is about

The optimized cooking extraction method was used in this study to extract gallus genomic DNA.OD260 /OD280 was up to 1.77±0.06 by the SmartspecTMplus test.The result showed that the quality of DNA extracted by the optimized cooking extraction method was very good,clear and bright fragments were detected through PCR amplification.Compared with the Phenol-chloroform extraction method,The optimized cooking extraction method is free from chloroform,phenol and other toxic reagents,and saves time and costs,hence a good method for extracting genomic DNA.

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Available abstract

The optimized cooking extraction method was used in this study to extract gallus genomic DNA.OD260 /OD280 was up to 1.77±0.06 by the SmartspecTMplus test.The result showed that the quality of DNA extracted by the optimized cooking extraction method was very good,clear and bright fragments were detected through PCR amplification.Compared with the Phenol-chloroform extraction method,The optimized cooking extraction method is free from chloroform,phenol and other toxic reagents,and saves time and costs,hence a good method for extracting genomic DNA.

Key concepts: genomic DNA, DNA extraction, Extraction (chemistry), DNA, Chloroform, Chromatography, Reagent, Polymerase chain reaction

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