2007•Zhongyi-yao xuebaoRequires access

Determination of rutin content in Xianji Huaban Capsules with HPLC

Che Xin-ying

Open publisher page 0 citations

Abstract

Objective:To establish a HPLC method for determination of rutin in Xianji Huaban capsules.Methods:The HPLC with the YWG-C18 column(4.6mm250mm,5μm) was used.The mobile phase was methanol-water-acetic acid(40:58:3) at a flow rate of 1.0ml·min-1,and the detective wavelength was at 360 nm.Results:There was a good linear correlation in the range of 0.105~0.525μg,r=0.9998.The recovery was 101.6%;RSD was 1.52%.Conclusion:The HPLC analysis method is simple and accurate and can be used for the quality control of the Capsules.

About this research paper

What this paper is about

Objective:To establish a HPLC method for determination of rutin in Xianji Huaban capsules.Methods:The HPLC with the YWG-C18 column(4.6mm250mm,5μm) was used.The mobile phase was methanol-water-acetic acid(40:58:3) at a flow rate of 1.0ml·min-1,and the detective wavelength was at 360 nm.Results:There was a good linear correlation in the range of 0.105~0.525μg,r=0.9998.The recovery was 101.6%;RSD was 1.52%.Conclusion:The HPLC analysis method is simple and accurate and can be used for the quality control of the Capsules.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To establish a HPLC method for determination of rutin in Xianji Huaban capsules.Methods:The HPLC with the YWG-C18 column(4.6mm250mm,5μm) was used.The mobile phase was methanol-water-acetic acid(40:58:3) at a flow rate of 1.0ml·min-1,and the detective wavelength was at 360 nm.Results:There was a good linear correlation in the range of 0.105~0.525μg,r=0.9998.The recovery was 101.6%;RSD was 1.52%.Conclusion:The HPLC analysis method is simple and accurate and can be used for the quality control of the Capsules.

Key concepts: Rutin, Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Acetic acid, Content determination, Linear relationship

Related papers

Back to paper searchBrowse research topicsOriginal source
Determination of rutin content in Xianji Huaban Capsules with HPLC — Research Paper | ScholarLens