Determination of rutin content in Xianji Huaban Capsules with HPLC
Che Xin-ying
Abstract
Che Xin-ying
Abstract
Objective:To establish a HPLC method for determination of rutin in Xianji Huaban capsules.Methods:The HPLC with the YWG-C18 column(4.6mm250mm,5μm) was used.The mobile phase was methanol-water-acetic acid(40:58:3) at a flow rate of 1.0ml·min-1,and the detective wavelength was at 360 nm.Results:There was a good linear correlation in the range of 0.105~0.525μg,r=0.9998.The recovery was 101.6%;RSD was 1.52%.Conclusion:The HPLC analysis method is simple and accurate and can be used for the quality control of the Capsules.
A significance statement is not available in the OpenAlex record.
A contribution statement is not available in the OpenAlex record.
Method details are not available in the OpenAlex metadata.
Findings are not separately available in the OpenAlex metadata.
Limitations are not available in the OpenAlex metadata.
Application details are not available in the OpenAlex metadata.
Objective:To establish a HPLC method for determination of rutin in Xianji Huaban capsules.Methods:The HPLC with the YWG-C18 column(4.6mm250mm,5μm) was used.The mobile phase was methanol-water-acetic acid(40:58:3) at a flow rate of 1.0ml·min-1,and the detective wavelength was at 360 nm.Results:There was a good linear correlation in the range of 0.105~0.525μg,r=0.9998.The recovery was 101.6%;RSD was 1.52%.Conclusion:The HPLC analysis method is simple and accurate and can be used for the quality control of the Capsules.
Key concepts: Rutin, Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Acetic acid, Content determination, Linear relationship