Determination of Kangnaoshuai capsules by HPLC
Liu Zhi-sheng
Abstract
Liu Zhi-sheng
Abstract
OBJECTIVE To establish an HPLC method for determination of Kangnaoshuai capsules.METHODS A Nova-pak C_18 column was used,the mobiLe phase was methanol-water (25∶75),detection wavelength was 250 nm.RESULTS The detection ranges were 10-100 mg·L~-1.The average recovery of puerarin was 97.76% (RSD=1.27%,n=5).CONCLUSION This HPLC method could be used for quaLity control in the Kangnaoshuai capsules.
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OBJECTIVE To establish an HPLC method for determination of Kangnaoshuai capsules.METHODS A Nova-pak C_18 column was used,the mobiLe phase was methanol-water (25∶75),detection wavelength was 250 nm.RESULTS The detection ranges were 10-100 mg·L~-1.The average recovery of puerarin was 97.76% (RSD=1.27%,n=5).CONCLUSION This HPLC method could be used for quaLity control in the Kangnaoshuai capsules.
Key concepts: Chromatography, High-performance liquid chromatography, Puerarin, Chemistry, Medicine, Alternative medicine, Pathology