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2006•The Chinese Journal of Modern Applied PharmacyRequires access

Determination of rutin in Maodongqing capsules by HPLC

Yilun Xu

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Abstract

OBJECTIVE To develop an HPLC method for the deformination of the rutin in Maodongqing capsules.METHODS The assay quantitative analysis was performed on a Kromasil C_(18) column with a mobile phase of methanol-water-glacial acetic acid(50∶48∶2),and detection wavelength at 360nm. RESULTS Good linearity of rutin was showed within the range of 5.14～(82.24)μg/mL(r=0.999 4).The average recovery was 98.74%,and RSD was 1.5%.CONCLUSION The method is simple and accurate.It is suitable for the quality control of this preparation.

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What this paper is about

OBJECTIVE To develop an HPLC method for the deformination of the rutin in Maodongqing capsules.METHODS The assay quantitative analysis was performed on a Kromasil C_(18) column with a mobile phase of methanol-water-glacial acetic acid(50∶48∶2),and detection wavelength at 360nm. RESULTS Good linearity of rutin was showed within the range of 5.14～(82.24)μg/mL(r=0.999 4).The average recovery was 98.74%,and RSD was 1.5%.CONCLUSION The method is simple and accurate.It is suitable for the quality control of this preparation.

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Available abstract

OBJECTIVE To develop an HPLC method for the deformination of the rutin in Maodongqing capsules.METHODS The assay quantitative analysis was performed on a Kromasil C_(18) column with a mobile phase of methanol-water-glacial acetic acid(50∶48∶2),and detection wavelength at 360nm. RESULTS Good linearity of rutin was showed within the range of 5.14～(82.24)μg/mL(r=0.999 4).The average recovery was 98.74%,and RSD was 1.5%.CONCLUSION The method is simple and accurate.It is suitable for the quality control of this preparation.

Key concepts: Rutin, Chromatography, High-performance liquid chromatography, Chemistry, Acetic acid, Methanol, Organic chemistry, Antioxidant

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