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The determination of baicalin in Xiaoerzhikeling Granule by HPLC

Fan Zhang

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Abstract

Objective: To establish a determination method of baicalin in Xiaoerzhikeling Granule.Method: HPLC,column: ODS-C_(18)(4.6 mm×250 mm,5 μm),mobile phase: methanol-0.4% phosphoric acid(45∶55,v/v),wavelength 280 nm.Result: The linear range was 0.122～0.732 μg(r=0.999 6) and recovery rate was 99.9%(RSD=0.56%).Conclusion: This method is convenient,sensitive and accurate.And it can be used as standard quality control of Xiaoerzhikeling Granule.

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What this paper is about

Objective: To establish a determination method of baicalin in Xiaoerzhikeling Granule.Method: HPLC,column: ODS-C_(18)(4.6 mm×250 mm,5 μm),mobile phase: methanol-0.4% phosphoric acid(45∶55,v/v),wavelength 280 nm.Result: The linear range was 0.122～0.732 μg(r=0.999 6) and recovery rate was 99.9%(RSD=0.56%).Conclusion: This method is convenient,sensitive and accurate.And it can be used as standard quality control of Xiaoerzhikeling Granule.

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Available abstract

Objective: To establish a determination method of baicalin in Xiaoerzhikeling Granule.Method: HPLC,column: ODS-C_(18)(4.6 mm×250 mm,5 μm),mobile phase: methanol-0.4% phosphoric acid(45∶55,v/v),wavelength 280 nm.Result: The linear range was 0.122～0.732 μg(r=0.999 6) and recovery rate was 99.9%(RSD=0.56%).Conclusion: This method is convenient,sensitive and accurate.And it can be used as standard quality control of Xiaoerzhikeling Granule.

Key concepts: Baicalin, Granule (geology), Chromatography, Phosphoric acid, High-performance liquid chromatography, Chemistry, Linear range, Quality standard

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