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2009•Chinese Journal of Ethnomedicine and EthnopharmacyRequires access

Determination of Baicalin in Xiaoerjiebiao Granule by HPLC

Song Zhi-zhao

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Abstract

Objective: To establish a method for the determination of baicalin in Xiaoerjiebiao granule.METHODS: HPLC method was used.The AICHROMA C18(4.6mm×250mm,5mm) was adopted as chromatographic column,the mobile phase consisted of methanol-water-phosphoric acid(50∶50∶0.2).The detection wavelength was at 280 nm and the column temperature was room temperature.RESULT: The calibration curve of baicalin was linear in the range of 10.02～50.10μg(r=0.9995),the average recovery rate was 100.6%,RSD=1.25%(n=5).CONCLUSIONS: The method is simple,accurate,reproducible and can be used as the Method of determination for the preparations.

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What this paper is about

Objective: To establish a method for the determination of baicalin in Xiaoerjiebiao granule.METHODS: HPLC method was used.The AICHROMA C18(4.6mm×250mm,5mm) was adopted as chromatographic column,the mobile phase consisted of methanol-water-phosphoric acid(50∶50∶0.2).The detection wavelength was at 280 nm and the column temperature was room temperature.RESULT: The calibration curve of baicalin was linear in the range of 10.02～50.10μg(r=0.9995),the average recovery rate was 100.6%,RSD=1.25%(n=5).CONCLUSIONS: The method is simple,accurate,reproducible and can be used as the Method of determination for the preparations.

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Available abstract

Objective: To establish a method for the determination of baicalin in Xiaoerjiebiao granule.METHODS: HPLC method was used.The AICHROMA C18(4.6mm×250mm,5mm) was adopted as chromatographic column,the mobile phase consisted of methanol-water-phosphoric acid(50∶50∶0.2).The detection wavelength was at 280 nm and the column temperature was room temperature.RESULT: The calibration curve of baicalin was linear in the range of 10.02～50.10μg(r=0.9995),the average recovery rate was 100.6%,RSD=1.25%(n=5).CONCLUSIONS: The method is simple,accurate,reproducible and can be used as the Method of determination for the preparations.

Key concepts: Baicalin, Chromatography, Calibration curve, Phosphoric acid, High-performance liquid chromatography, Granule (geology), Chemistry, Recovery rate

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