Determination of Three Tanshinones Content in Salviae miltiorrhizae Health Tea by HPLC

[Objective]To establish a HPLC method for simultaneously determining three major tanshinones in Salviae miltiorrhizae health tea.[Method]HPLC was employed to determine the content of each composition.The HPLC conditions were as follows: column of Insertsil ODS-SP(4.6 mm×250 mm,5 μm),mobile phase of a mixture of acetonitrile-water(V∶V=62∶38),detection wavelength of 270 nm,flow rate of 1.0 ml/min,sampling amount of 15 μl and column temperature of 30 ℃.[Result]Three compositions were well-separated and the average recovery of cryptotanshinone,tanshinone Ⅰ,tanshinone Ⅱ A were 98.2%,98.7% and 98.4% respectively(RSD value of 1.20%,1.96% and 1.74%,n=3).The content of the tanshinones in health tea were 1.19,0.79 and 2.25 mg/g respectively.[Conclusion]The method is simple and rapid,which can be used to control the quality of Salviae miltiorrhizae health tea.