Determination of Icariin in Funingkang Soft Capsule by HPLC

Objective: To establish a method for the determination of icariin in Funingkang soft capsules. Methods: HPLC was used to determine the icariin in Funingkang soft capsules. Diamonsil (TM) column (4.6 × 150 mm, 5 μm) was used as analytical column with the column temperature set at 25 ℃. The mobile phase of icariin was acetonitrile-water-HAc (27:75:0.6) and the UV detective wavelength was 270 nm. The flow rate was 1.0 mL·min-1. Results: The calibration curves of icariin were linear in the ranges of 3- 18 μg· mL-1 (r = 0.999 9), and the average recoveries of icariin was 100.3 % (RSD = 2.27 %). Conclusion: The method is simple and accurate, with good reproducibility. It can be used as the quality control of icariin in Funingkang soft capsules.