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Content Determination of Icariin in Zhuangguling Capsules

Lianzhi Wang

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Abstract

Objective: To establish a method of HPLC for determination of icariin in Zhuangguling capsules.Methods: The HPLC column was Diamonsil C18(5μm,250×4.6mm).The mobile phase consisted of acetonitrile-water(30∶ 70) at the flow rate of 1.0ml/min.The column temperature was 40℃.The detection wavelength was set as 270nm.Results: The linear range of icariin was 0.1ug~0.18ug(r=0.999 9),and the average recovery was 102.4%(RSD=1.35%).Conclusion:The method is accurate,reliable and reproducible,and it can be used as the content determination for Zhuangguling capsules.

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What this paper is about

Objective: To establish a method of HPLC for determination of icariin in Zhuangguling capsules.Methods: The HPLC column was Diamonsil C18(5μm,250×4.6mm).The mobile phase consisted of acetonitrile-water(30∶ 70) at the flow rate of 1.0ml/min.The column temperature was 40℃.The detection wavelength was set as 270nm.Results: The linear range of icariin was 0.1ug~0.18ug(r=0.999 9),and the average recovery was 102.4%(RSD=1.35%).Conclusion:The method is accurate,reliable and reproducible,and it can be used as the content determination for Zhuangguling capsules.

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Available abstract

Objective: To establish a method of HPLC for determination of icariin in Zhuangguling capsules.Methods: The HPLC column was Diamonsil C18(5μm,250×4.6mm).The mobile phase consisted of acetonitrile-water(30∶ 70) at the flow rate of 1.0ml/min.The column temperature was 40℃.The detection wavelength was set as 270nm.Results: The linear range of icariin was 0.1ug~0.18ug(r=0.999 9),and the average recovery was 102.4%(RSD=1.35%).Conclusion:The method is accurate,reliable and reproducible,and it can be used as the content determination for Zhuangguling capsules.

Key concepts: Icariin, Chromatography, High-performance liquid chromatography, Linear range, Content determination, Chemistry, Linear relationship, Recovery rate

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