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2004•Lishizhen Medicine and Materia Medica ResearchRequires access

Experimental Study of Salidroside Against Hepatic Fibrosis in Vitro

Wang Xiao

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Abstract

Objective:To investigate the effects of salidroside on proliferation and extracellular matrix secretion of rat hepatic stellate cells in vitro.Methods:HSC-T6 were incubated with different concentration of salidroside for 24 hours.MTT colorimetry was used for assaying proliferation of HSC.Cytotoxicity was measured by LDH colorimetry.Type Ⅰ collage was determined by ELISA.Hyaluronic Acid and Laminin were measured by Radioimmunoassay.Results:Addition of 1～8 mg/ml salidroside into culture medium had no cytotoxicity to HSC, but could signficantly inhibit HSC proliferation, and also inhibit Type Ⅰ collage, Hyaluronic Acid and Laminin secretion in different degree.Conclusion:Salidroside has significant effects on hepatic fibrosis in vitro.

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What this paper is about

Objective:To investigate the effects of salidroside on proliferation and extracellular matrix secretion of rat hepatic stellate cells in vitro.Methods:HSC-T6 were incubated with different concentration of salidroside for 24 hours.MTT colorimetry was used for assaying proliferation of HSC.Cytotoxicity was measured by LDH colorimetry.Type Ⅰ collage was determined by ELISA.Hyaluronic Acid and Laminin were measured by Radioimmunoassay.Results:Addition of 1～8 mg/ml salidroside into culture medium had no cytotoxicity to HSC, but could signficantly inhibit HSC proliferation, and also inhibit Type Ⅰ collage, Hyaluronic Acid and Laminin secretion in different degree.Conclusion:Salidroside has significant effects on hepatic fibrosis in vitro.

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Available abstract

Objective:To investigate the effects of salidroside on proliferation and extracellular matrix secretion of rat hepatic stellate cells in vitro.Methods:HSC-T6 were incubated with different concentration of salidroside for 24 hours.MTT colorimetry was used for assaying proliferation of HSC.Cytotoxicity was measured by LDH colorimetry.Type Ⅰ collage was determined by ELISA.Hyaluronic Acid and Laminin were measured by Radioimmunoassay.Results:Addition of 1～8 mg/ml salidroside into culture medium had no cytotoxicity to HSC, but could signficantly inhibit HSC proliferation, and also inhibit Type Ⅰ collage, Hyaluronic Acid and Laminin secretion in different degree.Conclusion:Salidroside has significant effects on hepatic fibrosis in vitro.

Key concepts: Salidroside, Hyaluronic acid, Hepatic stellate cell, Colorimetry, In vitro, Chemistry, Cytotoxicity, Laminin

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