GC-MS Analysis and Determination of Fatty Acids in Bee Pollen

The crude fat was extracted from 8 kinds of bee pollen such as camellia pollen, poppy pollen, peach pollen, lotus flower pollen, amboyna pollen, watermelon pollen, dandelion pollen, broad beans pollen through Soxhlet extraction. The contents of crude fat and free fatty acids in bee pollen were also determined. The relative contents of fatty acids were further analyzed and determined via GC-MS after esterification of crude extracts. Results indicated that 8 kinds of bee pollen had plentiful fatty acids, which were important function factors for nutrition.