Amplification and cloning of Glutathione-S-Transferase gene of Schistosoma japinicum (Chinese Stain)

SjcDNA first strand synthesis was driven by superscript Ⅱ RT using adult worm RNA as template.Specific primers was designed and synthesized.Coding region gene of SjGST was amplified by PCR.The product from PCR was cloned into pGEM-T-GST vector.The cloning vector pGEM-T-GST was identified by restriction analysis and PCR.The results demonstrated that pGEM-T-GST was successfully constructed and provided the basis for further study on GST expression and its immunological diagnosis and prevention.