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2000•Academic Journal of Sun Yat-sen University of Medical SciencesRequires access

Cloning of 26 ku Glutathione S-transferase Gene of Schistosoma japonicum and Analysis

Shuhong Luo

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Abstract

Objective To amplify the gene of 26 ku glutathione S-transferase of Schistosoma japonicum (Sj26GST) and construct a recombinant prokaryotic expression vector. Methods One pair of primers was designed according to the sequence of GST. The cDNA fragment of Sj26GST gene was obtained by RT-PCR, then it was cloned into a vector -pBluescript(KS),and corroborated through restriction enzymes map、PCR and sequencing. Results GST-pBluescript(KS) recombinant clone was constructed successfully. Conclusions GST recombinant clone was achieved in the experiment. It is prepared for further study on Gene-engineering vaccine.

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What this paper is about

Objective To amplify the gene of 26 ku glutathione S-transferase of Schistosoma japonicum (Sj26GST) and construct a recombinant prokaryotic expression vector. Methods One pair of primers was designed according to the sequence of GST. The cDNA fragment of Sj26GST gene was obtained by RT-PCR, then it was cloned into a vector -pBluescript(KS),and corroborated through restriction enzymes map、PCR and sequencing. Results GST-pBluescript(KS) recombinant clone was constructed successfully. Conclusions GST recombinant clone was achieved in the experiment. It is prepared for further study on Gene-engineering vaccine.

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Available abstract

Objective To amplify the gene of 26 ku glutathione S-transferase of Schistosoma japonicum (Sj26GST) and construct a recombinant prokaryotic expression vector. Methods One pair of primers was designed according to the sequence of GST. The cDNA fragment of Sj26GST gene was obtained by RT-PCR, then it was cloned into a vector -pBluescript(KS),and corroborated through restriction enzymes map、PCR and sequencing. Results GST-pBluescript(KS) recombinant clone was constructed successfully. Conclusions GST recombinant clone was achieved in the experiment. It is prepared for further study on Gene-engineering vaccine.

Key concepts: Schistosoma japonicum, Recombinant DNA, Glutathione S-transferase, Molecular biology, clone (Java method), Biology, Cloning (programming), Complementary DNA

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