Molecular Cloning and Sequence Analysis of Glutathione S-transferase Gene in Schistosoma japonicum

In order to construct the recombinant clone of glutathione S-transferase gene(GST) in Schistosoma japonicum and express the recombinant sjGST protein with his-tag that could be purified by single-step Ni-NTA affinity chromatography,the DNA fragment of sjGST gene was amplified by PCR with template PGEX-KG.Then it was cloned into vector pET28a,and the recombinant prokaryotic expressing vector was constructed and corroborated through restriction enzymes map and sequencing.Identity analysis and the primary and tertiary structure of translation alignment were made by using NCBI-blast and Swiss-module.Results showed that pET28a-sjGST recombinant clone was constructed successfully.The length of amplified GST gene has the opening reading frame(ORF) of 687 bp in length and has 99% identity with PGEX-KG-GST,S.japonicum-GST-Mainland-26ku(sjGST-ML-26) and S.japonicum-GST-Philippines-26ku(sjGST-PL-26).