Amplification and cloning of 31/32 KDa proteins gene of Schistosoma japonicum

Objecitve To clone 31/32 KDa proteins gene of Schistosoma japoninicum in order to study its effect on immunological diagnosis and prevention. Methods Synthesis of the first SjcDNA strand was driven by superscript Ⅱ RT using adult worm RNA as template. Specific primers was designed and synthesized according to mRNA sequence of S.m 31/32KDa gene. Coding region gene of Sj 31/32 KDa proteins was amplified by PCR. The product from PCR was cloned into pGEM T 31/32 vector which was identified later by restriction analysis and PCR. Results For RT PCR, a specific band about 1 270 bp was amplified. The same band was obtained by double restriction of recombinant plasmids and PCR of using recombinant plasmids as template. Conclusions pGEM T 31/32 KDa was successfully constructed and provided as the basis for further studying on 31/32 KDa proteins expression and its function.