2012•Xiandai shengwu yixue jinzhanRequires access

The Content Determination of Paeonol in Shuxin Pills by HPLC

Zhenyu Wu

Abstract

Objective:To establish the content determination method of paeonol in Shuxin Pills.Methods:The quantitative analysis of paeonol in Shuxin Pills was established by HPLC with Kromsal C18 chromatographic column(250mm×4.6mm,5 μ m), mobile phase methanol-water(60 :40),1.0 mL/min flow rate,274nm detection wavelength.Results:The linear ranges were 0.021-0.105 mg/ml with good positive correlation.r was 0.9997.The average recovery rate was 99.07% and RSD was 0.36%.Conclusion:The established methods are accurate,exclusive,reproducible and suitable for the quality control of paeonol in Shuxin Pills.

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Key concepts: Paeonol, Chromatography, High-performance liquid chromatography, Content determination, Chemistry, Linear correlation, Pill, Linear relationship