2012•Journal of clinical and experimental medicineRequires access

Study on determination of paeonol content in Liuwei Dihuang pills(condensed) by HPLC

Luo Guo-hu

Abstract

Objective To establish a HPLC method for determination of paeonol content in Liuwei Dihuang pills(condensed).Methods The chromatic column of C18 was applied.The mobile phase consisted of methanol-water(70:30);the wave length for detection was at 274 nm;flow rate was kept at 1.0 ml/min.The column temperature kept at room temperature.Results The determination of paeonol content in HPLC had good linear relation in ranges of 8.24～164.8 μg/ml(r=0.9999),average recovery rate was 98.92%,and relative standard deviation(RSD)=1.70%(n=6).Conclusion The results showed that HPLC is convenient,fast,accurate and reproducible in determination of paeonol content in Liuwei Dihuang pills(condensed).It can be used for controlling the quality of this kind of product of Chinese traditional medicine.

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Key concepts: Paeonol, Chromatography, High-performance liquid chromatography, Content determination, Medicine, Chemistry, Alternative medicine, Pathology