Inhibition of Qiyaoxiaoke capsule on the glomerular mesangial cell proliferation induced by high glucose

Objective To observe the inhibition of Qiyaoxiaoke capsule on the glomerular mesangial cell(GMCs) proliferation induced by high glucose.Methods Different concentration serum was prepared by serum pharmacological approach of containing drug.Optimum concentration of glucose was selected by mesangial cells in vitro culture techniques.The glomerular mesangial cell proliferation under different times and different concentrations were observed by MTT.Results The cell growth in blank group is the slowest at different time points.Compared with the control group,in the same culture conditions,GMCs proliferation stimulation in 0.4 g/L glucose group and 0.5 g/L glucose group at 48 h,72 h were stronger(P0.05,P0.01),GMCs proliferation stimulating effect in 0.6 g/L glucose group,0.7 g/L glucose group and 0.8 g/L glucose group at 24 h,48 h,72 h were stronger(P0.05,P0.01),GMCs proliferation stimulating effect in 0.6 g/L glucose at 48 h,72h were the strongest(P0.01).GMCs proliferation in high glucose group was more rapid than that in blank control group(P0.05,P0.01).Compared with the high glucose group,GMCs proliferation in each group of containing Qiyaoxiaoke capsule serum at 48 h,72 h was slow(P0.05).Conclusion Qiyaoxiaoke capsule has inhibition on the glomerular mesangial cell proliferation.