2004•Zhongguo xiandai yixue/Zhongguo xiandai yixue zazhiRequires access
Influence of high glucose and other factors on negative cell regulatory protein p27 on glomerular mesangial cells
Hui Qi, Furong Li, Xingen Wang, Ren Lili, Ruifang Huang, Yong Dai
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Abstract
Objective: To observe the change of negative cell-cycle regulatory protein p27 expression on glomerular mesangial cells under the influence of high glucose, ET-1 and IL-13. Methods: Glucose (5.5 mmol/L and 25 mmol/L), ET-1(10-7 mol/L) or IL-13(10 ng/ml)was added into the medium of cultured glomerular mesangial cells separately. The expression level of p27 was assayed by flow cytometry. Results: Compared with control group, the level of p27 in glomerular mesangial cells of glucose 5.5 mmol/L group at 48 h (5.14±0.94) and 72 h (3.84±0.81) decreased significantly (P0.001), glucose 25 mmol/L group (26.82±3.15 and 30.88±3.68) increased significantly (P0.05 at 72 h), of ET-1 group at 14 h and 18 h (14.76±1.49 and 12.18±1.30) decreased significantly (P0.05, P0.01), IL-13 group at 48 h (46.74±3.25) increased significantly (P0.001. Conclusion: Negative cell-cycle regulator p27 plays a very important role in regulating the proliferation of glomerular mesangial cells.