Effects of Baicalin of Down-Regulation of NF-κB Expression and the Inhibition on Glomerular Mesangial Cell Proliferation Induced by High Glucose

Objective To study the inhibition of baicalin on glomerular mesangial cell(GMC)proliferation induced by high glucose and the impact on NF-κB expression.Methods The in vitro cultured rat GMCs were divided into six groups,named a low-glucose group,a high-glucose group,a low-dose baicalin group,a middle-dose baicalin group,a high-dose baicalin group and a Losartan group,cultured for 24 h,48 h and 72 h separately.At the end of experiment,according to MTT detection results,the cells were collected and the nuclear protein was extracted.Western blot method was adopted to detect NF-κB expression in each group.Results In the high-glucose group,the absorption value in 24 h,48 h and 72 h was increased as compared with the low-glucose group separately,which was getting apparent with the passing of culture time(P0.05).In all of the low-dose,middle-dose and high-dose baicalin groups and the Losartan group,the absorption value in 24 h was higher than that in the high-glucose group,but the result in 48 h and 72 h was lower than that in the high-glucose group(P0.05).Western blot results showed that NF-κB expression in the high-glucose group was increased as compared with the low-glucose group.After the intervention with baicalin,NF-κB expression in GMCs was reduced as compared with the high-glucose group,which was gradually reduced along with the concentration increased.Conclusion Under the high glucose condition,the abnormal proliferation happens in the in vitro cultured GMCs.Baicalin inhibits GMC abnormal proliferation induced by high glucose,indicating the time-dose dependent relationship.The mechanism of it is probably related to the down-regulation of NF-κB expression achieved by baicalin.