Effects of L-EGCG on proliferation glomerular mesangial cells of rat model induced by lipoprotein(a).

Objective To investigate the effects of L-epigallocatechins gallic acid ester(L-EGCG)on proliferation of glomerular mesangial cells(GMCs)of rat model induced by lipoprotein(a)[Lp(a)] and explore the possible mechanism of L-EGCG on the proliferation of rat GMCs by Lp(a).Methods Rat GMCs were divided into three groups:control group,Lp(a)(5.0μg/ml)group,Lp(a)(5.0μg/ml)+ L-epigallocatechins gallic acid ester(L-EGCG 200mg/L)group.After culture(at the end of 12h,24h,48h,60h and 72h),collecting the cultured GMCs and suspension to observe the rate of GMCs proliferation(MTT),the positive rate of proliferation cell nuclear antigen(PCNA)(immuno-histo-chemisty),and to detect intercellular adhesion molecule-1(ICAM-1)activity(ELISA).Results At the end of experiment,MTT,the positive rate of PCNA and ICAM-1 activity of GMCs were increased as culture time increased in three group.Compared with control group,MTT,positive rate of PCNA and ICAM-1 activity of GMCs were more significantly enhanced in Lp(a)group.Compared with Lp(a)group,MTT,positive rate of PCNA and ICAM-1 activity of GMCs were significantly decreased in Lp(a)+L-EGCG group.ICAM-1 activity showed positive correlationship with MTT and the positive rate of PCNA.Conclusion Lp(a)can significantly affect the rate of GMCs proliferation.L-EGCG can depress proliferation GMCs of rat model induced by Lp(a)and correct with ICAM-1.