2004•PubMedRequires access

[Screening of cDNA library of Schistosoma japonicum with sera from rabbits vaccinated with ultraviolet-attenuated schistosomula].

Xiaohong Li, Shuxian Liu, Guang-cheng Song, Yu-xin Xu, Jianping Cao, Jiaxu Chen

Abstract

OBJECTIVE: To search new potential schistosomiasis vaccine by screening cDNA library with sera of rabbits vaccinated with attenuated larvae. METHODS: Schistosoma japonicum (Sj) adult worm cDNA library was screened with sera of rabbits vaccinated with ultraviolet-attenuated schistosomula and by sera of infected rabbits, and the inserts of positive clones were amplified and sequenced. RESULTS: Six kinds of Sj genes were obtained after three rounds of screening. Among the genes were glyceraldehyde 3-phosphate dehydrogenase (GAPDH), serine protease inhibitors (serpin), mitochondrion coding region, part of myosin heavy chain gene and two new genes, respectively. CONCLUSION: Screening cDNA library with sera of animals vaccinated with attenuated larvae is an effective way to search new vaccine candidates for schistosomiasis.

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Key concepts: Schistosoma japonicum, cDNA library, Complementary DNA, Biology, Virology, Molecular biology, Gene, Attenuated vaccine