2003•PubMedRequires access

[Schistosoma japonicum: screening of cDNA library with sera from self-cured water buffaloes (Bos buffelus)].

Wenqin Wang, Shuxian Liu, Guang-cheng Song, Yu-xin Xu, Jianping Cao, Jiaxu Chen

Abstract

OBJECTIVE: To understand and identify the molecules related to the self-cure of Schistosoma japonicum infection in water buffaloes. METHODS: The S. japonicum adult worm cDNA library was immunologically screened with the sera of self-cured water buffaloes. The positive clones were identified, cloned, sequenced and analysed with software. RESULTS: Three genes encoding antigens relevant to sera antibodies in water buffaloes were cloned and sequenced. These antigens included paramyosin (Sj97), GST, carbonyl reductase-like 20-beta-hydroxysteroid dehydrogenase(CR/20-beta-HSD). CONCLUSION: Sera from self-cured S. japonicum infected water buffaloes can be used to screen adult worm cDNA library for vaccine development.

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Key concepts: Schistosoma japonicum, cDNA library, Complementary DNA, Biology, Antigen, Gene, Antibody, Schistosoma