Resistance and analysis of resistant genes of multidrug-resistant Pseudomonas aeruginosa

Objective To investigate the resistant genes of multidrug-resistant Pseudomonas aeruginosa isolated from Respiratory Medicine Ward.Method The antimicrobial susceptibility was evaluated by disk diffusion test.The genes TEM,SHV,CTX-M-9,DHA,VIM,PER,OXA,IMP and OprD2 carried on 32 strains of multidrug-resistant Pseudomonas aeruginosa were detected by polymerase chain reaction(PCR),and the resistant gene sequences were identified.Result The resistance rate of 32 Pseudomonas aeruginosa isolates to polymyxin B was 3.1%,and the resistance rates to other antibiotics in between 53% to 100%.The positive rates of OXA-10,TEM-1,DHA-1,PER-1 and IMP-1 among β-laetamase genes were 46.9%,21.9%,15.6%,12.5% and 31.3% respectively.SHV,CTX-M-9 and VIM genes were not found.The loss rate of OprD2 gene was 68.8%.Conclusion The multidrug-resistant Pseudomonas aeruginosa carrying a variety of β-lactamase from Respiratory Medicine Ward should be paid much attention.