Genic analysis of multidrug resistant Pseudomonas aeruginosa in nosocomial infection

Objective To investigate the multidrug resistance and resistant mechanisms of Pseudomonas aeruginosa in nosocomial infection.Methods Thirty-seven strains of Pseudomonas aeruginosa were performed antimicrobial susceptibility test with microdilution broth test method,the beta-lactamase gene,aminoglycosides modifying enzyme gene,disinfectans and sulfonamides-resistant gene carried on multidrug resistant Pseudomonas aeruginosa were detected by polymerase chain reaction method,and the resistant gene sequences were identified.Results The positive rate of β-lactamase genes TEM,CARB,VIM expression was 24.32%(9/37),43.24%(16/37) and 5.41%(2/37) respectively,while the expression of SHV,CTX-M,OXA-10group,PER,GES,VEB,DHA and IMP were not detectable.The absent rate of oprD2 was 86.49%(32/37).The positive rate of aminoglycosides modifying enzyme genes aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(3″)-Ⅰand ant(2″)-Ⅰexpression was 8.11%(3/37),43.24%(16/37),18.92%(7/37) and 40.54%(15/37) respectively.The positive rate of disinfectants and sulfonamides-resistant gene qacE△1-sul1 expression was 51.35%(19/37).Conclusion Pseudomonas aeruginosa involved in nosocomial infection carrys multidrug resistant genes,which severely affects clinical anti-infection therapy.