Study on related resistant genes of multi-resistant Pseudomonas aeruginosa from Yunfu area

Objective To study the related resistant genes in multidrug-resistant Pseudomonas aeruginosa isolated from Yunfu area.Methods The Kirby-Bauer tests were performed to detect the susceptibility of 18 kinds of antimicrobial agents against 38 strains of Pseudomonas aeruginosa.The β-lactamases gene and aminoglycosides modifying enzymes gene carried on multidrug resistant Pseudomonas aeruginosa were detected by polymerase chain reaction method,and the resistant gene sequences were identified.Results The resistance rates of 25 Pseudomonas aeruginosa isolates to polymyxin B was 0%,the resistance rate of other antimicrobial agents was more than 50%.The positive rate of ant(2″)-Ⅰ,ant(3″)-Ⅰ,aac(6′)-Ⅰ,aac(6′)-Ⅱand aac(3)-Ⅱamong aminoglycosides-modifying enzymes genes in 25 multidrug-resistant Pseudomonas aeruginosa was 20%,32%,24%,28% and 24%,respectively.aac(3)-Ⅰ genes was not found.The positive rate of OXA-10,TEM-1,SHV,DHA-1,PER and IMP-9 among β-lactamases genes in 25 multidrug-resistant Pseudomonas aeruginosa was 32%,12%,8%,12%,12% and 12% respectively.CTX-M-9 and VIM genes was not found.The loss rate of OprD2 genes was 60%.Conclusion The multidrug-resistant Pseudomonas aeruginosa carrying a variety of β-lactamases and aminoglycosides-modifying enzyme gene,which should be paid attention to.