2010•Chinese Journal of Health Laboratory TechnologyRequires access
Genic analysis of multidrug-resistant Pseudomonas aeruginosa
Huaying Mao
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Abstract
Objective:To investigate beta-lactamase genes,outer membrance protein D2(OprD2)genes,aminoglycoside-modifying enzyme genes,resistant disinfectant genses in Multidrug-resistant Pseudomonas aeruginosa separated from clinical specimens in our hospital.Methods:The antibiotic susceptibility of 17 different antibiotics of Pseudomonas aeruginosa was tested by K-B methord,and 100 multidrug-resistant strains were screened.Beta-lactamase genes,OprD2 genes,aminoglycoside-modifying enzyme genes and qacE△1-sul1 gene were detected by polymerasechain reaction(PCR).Results:Among the 100 strains of multidrug-resistant Pseudomonas aeruginosa,the detection rate of Beta-lactamase gene CTX-M-1 group,TEM and DHA was 54.0%,30.0% and 9.0% respectively,and none of the 100 strains possessed genes of SHV,OXA-10 group,PER,GES,VEB,CARB,IMP and VIM.61 strains(61.0%) lost OprD2 gene.The detection rate of Aminoglycoside-modifying enzyme gene aac(6′)-Ⅱ,ant(3″)-Ⅰ and ant(2″)-Ⅰwas 59.0%,33.0% and 25.0% respectively,none possessed gene of aac(6′)-Ⅰ.The detection rate of qacE△1-sul1 was 15.0%.Conclusion:Multidrug-resistant Pseudomonas aeruginosa in our hospital carrys Multidrug resistant genes,resistance to beta-lactam compounds in Multidrug-resistant Pseudomonas aeruginosa of our hospital is related to CTX-M-1 group,TEM and DHA genes.Resistance to imipenem is related to the loss of OprD2 gene.Resistance to aminoglycosides is related to aac(6′)-Ⅱ,ant(3″)-Ⅰ and ant(2″)-Ⅰ genes.Resistance to disinfectant is related to qacE△1-sul1 gene.