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2011•Liaoning Zhongyiyao Daxue xuebaoRequires access

Determination of Paeoniflorin in Shaofu Zhuyu Granule by HPLC

Wei Wang

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Abstract

Objective:To determine Paeoniflorin in shaofuzhuyu Granule.Methods:Agilent 1100 HPLC and SHIMADZU C18 column(4.6mm×150mm)was eluted,the mobile phase consisted of methanol-water at the column temperature 30℃.The flow rate was 0.9mL/min and detective wavelength was 230nm.Results:Paeoniflorin was proved to be linear in the range of 0.1202~0.7212μg with a regression coefficient of 0.9996.The average recovery was 98.9%(n=6,RSD=0.53%).Conclusion:This method was sensitive,quick and accurate for the quality control of this preparation.

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What this paper is about

Objective:To determine Paeoniflorin in shaofuzhuyu Granule.Methods:Agilent 1100 HPLC and SHIMADZU C18 column(4.6mm×150mm)was eluted,the mobile phase consisted of methanol-water at the column temperature 30℃.The flow rate was 0.9mL/min and detective wavelength was 230nm.Results:Paeoniflorin was proved to be linear in the range of 0.1202~0.7212μg with a regression coefficient of 0.9996.The average recovery was 98.9%(n=6,RSD=0.53%).Conclusion:This method was sensitive,quick and accurate for the quality control of this preparation.

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Available abstract

Objective:To determine Paeoniflorin in shaofuzhuyu Granule.Methods:Agilent 1100 HPLC and SHIMADZU C18 column(4.6mm×150mm)was eluted,the mobile phase consisted of methanol-water at the column temperature 30℃.The flow rate was 0.9mL/min and detective wavelength was 230nm.Results:Paeoniflorin was proved to be linear in the range of 0.1202~0.7212μg with a regression coefficient of 0.9996.The average recovery was 98.9%(n=6,RSD=0.53%).Conclusion:This method was sensitive,quick and accurate for the quality control of this preparation.

Key concepts: Paeoniflorin, Granule (geology), Chromatography, Medicine, High-performance liquid chromatography, Composite material, Chemistry, Materials science

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