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2008•Guiding Journal of Traditional Chinese Medicine and PharmacyRequires access

Determination of Paeoniflorin in Egui-Yangxue Granule by HPLC

Yang Li-ping

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Abstract

Objective:To establish a HPLC method for the determination of Paeoniflorin in Egui-yangxue Granule.Methods:Diamonsil C18(4.6 mm×250 mm)column was used,the mobile phase was a mixed liquid with acetonitrile-0.05%H3PO4(16∶84).The detection wavelength was at 230 nm and the flow rate was 1mL/min.Results:A good linearity of Paeoniflorin was in the range of 92.5-740 ng and the average recovery was 99.32%.Conclusion: The method is rapid,sensitive and accurate.It can be used to controi the quality of Egui yangxue Granule.

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What this paper is about

Objective:To establish a HPLC method for the determination of Paeoniflorin in Egui-yangxue Granule.Methods:Diamonsil C18(4.6 mm×250 mm)column was used,the mobile phase was a mixed liquid with acetonitrile-0.05%H3PO4(16∶84).The detection wavelength was at 230 nm and the flow rate was 1mL/min.Results:A good linearity of Paeoniflorin was in the range of 92.5-740 ng and the average recovery was 99.32%.Conclusion: The method is rapid,sensitive and accurate.It can be used to controi the quality of Egui yangxue Granule.

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Available abstract

Objective:To establish a HPLC method for the determination of Paeoniflorin in Egui-yangxue Granule.Methods:Diamonsil C18(4.6 mm×250 mm)column was used,the mobile phase was a mixed liquid with acetonitrile-0.05%H3PO4(16∶84).The detection wavelength was at 230 nm and the flow rate was 1mL/min.Results:A good linearity of Paeoniflorin was in the range of 92.5-740 ng and the average recovery was 99.32%.Conclusion: The method is rapid,sensitive and accurate.It can be used to controi the quality of Egui yangxue Granule.

Key concepts: Paeoniflorin, Chromatography, Granule (geology), High-performance liquid chromatography, Chemistry, Acetonitrile, Materials science, Composite material

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