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2011•Guide of China MedicineRequires access

Determination of Paeoniflorin in Gonghuan Yangxue Granule by HPLC

Yuehua Deng

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Abstract

Objective To establish a HPLC for determination of Paeoniflorin in Gonghuan Yangxue Granule.Method A Shim-pack VP-ODS column was used.The mobile phase was acetonitrile-0.05%phosphate(16∶84),the detecting wavelength was at 230nm,the flow rate was 1.0mL/min.Result A good linearity of Paeoniflorin was in the range of 0.0476-0.912μg,r=0.9996,the average sample recovery was 99.51% with RSD of 1.35%(n=6).Conclusion This method has good repeatability and flexibility.It can be used for quality control of Gonghuan Yangxue Granule.

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What this paper is about

Objective To establish a HPLC for determination of Paeoniflorin in Gonghuan Yangxue Granule.Method A Shim-pack VP-ODS column was used.The mobile phase was acetonitrile-0.05%phosphate(16∶84),the detecting wavelength was at 230nm,the flow rate was 1.0mL/min.Result A good linearity of Paeoniflorin was in the range of 0.0476-0.912μg,r=0.9996,the average sample recovery was 99.51% with RSD of 1.35%(n=6).Conclusion This method has good repeatability and flexibility.It can be used for quality control of Gonghuan Yangxue Granule.

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Available abstract

Objective To establish a HPLC for determination of Paeoniflorin in Gonghuan Yangxue Granule.Method A Shim-pack VP-ODS column was used.The mobile phase was acetonitrile-0.05%phosphate(16∶84),the detecting wavelength was at 230nm,the flow rate was 1.0mL/min.Result A good linearity of Paeoniflorin was in the range of 0.0476-0.912μg,r=0.9996,the average sample recovery was 99.51% with RSD of 1.35%(n=6).Conclusion This method has good repeatability and flexibility.It can be used for quality control of Gonghuan Yangxue Granule.

Key concepts: Paeoniflorin, Medicine, Chromatography, Granule (geology), Repeatability, High-performance liquid chromatography, Composite material, Materials science

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