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2001•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Determination of Paeoniflorin in Yigankang Capsules by High Performance Liquid Chromatography

Xi Liu

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Abstract

Aim To establish a RP-HPLC method for determination of Paeoniflorin in Yigankan Capsules.Method The contents of Paeoniflorin was determined by HPLC, using Spherisorb C 18 (5μm, 4.6×200mm)column, methanol-0.05mol·L -1 Natrium Dihydrogen Phosphate-Isopropanol(40∶65∶2) as the mobile phase. The detective wavelength was 230nm.Results Paeoniflorin at the concentrations of 0.5～10μg showed good linearity(r=0.9998).The average recovery was 98.66% with the relative standard deviationRSD 1.88%(n=5). Conclusion The results show that the method is simple and accurate. It can be useful in quality control of the products.

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Aim To establish a RP-HPLC method for determination of Paeoniflorin in Yigankan Capsules.Method The contents of Paeoniflorin was determined by HPLC, using Spherisorb C 18 (5μm, 4.6×200mm)column, methanol-0.05mol·L -1 Natrium Dihydrogen Phosphate-Isopropanol(40∶65∶2) as the mobile phase. The detective wavelength was 230nm.Results Paeoniflorin at the concentrations of 0.5～10μg showed good linearity(r=0.9998).The average recovery was 98.66% with the relative standard deviationRSD 1.88%(n=5). Conclusion The results show that the method is simple and accurate. It can be useful in quality control of the products.

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Available abstract

Aim To establish a RP-HPLC method for determination of Paeoniflorin in Yigankan Capsules.Method The contents of Paeoniflorin was determined by HPLC, using Spherisorb C 18 (5μm, 4.6×200mm)column, methanol-0.05mol·L -1 Natrium Dihydrogen Phosphate-Isopropanol(40∶65∶2) as the mobile phase. The detective wavelength was 230nm.Results Paeoniflorin at the concentrations of 0.5～10μg showed good linearity(r=0.9998).The average recovery was 98.66% with the relative standard deviationRSD 1.88%(n=5). Conclusion The results show that the method is simple and accurate. It can be useful in quality control of the products.

Key concepts: Paeoniflorin, Chromatography, High-performance liquid chromatography, Chemistry, Relative standard deviation, Methanol, Detection limit, Organic chemistry

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