Study on purifying the total alkaloids in Atalantia buxifolia with AB-8 macroporous resin

Objective To study on purifying the total alkaloids in Atalantia buxifolia with macroporous resin.Methods The content of total alkaloids were determined by acid dye-colorimetry. The content of total alkaloids as index,the type of macroporous resin,sample volume,concentration,p H and volume of elution solvent were optimized by single-factor experiments. Results Optimal purification process conditions were as follows: optimal macroporous resin type AB-8,the concentration of sample solution 0.14 g·m L-1with p H as 10.0,maximum sample volume 5 BV,washed by 9 BV purified water and 10 BV 95% ethanol,collected eluent of 95% ethanol. The average transfer rate of total alkaloids was 83.61%,and the adsorption and desorption rate were 89. 30% and 93. 64%,respectively. The RSD was 0. 30%. Conclusion The total alkaloids in Atalantia buxifolia can be purified effectively by AB-8 macroporous resin with good reproducibility.